Human/Rat Cortactin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6096
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Rat
Applications
Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Cortactin
Met1-Gly85
Accession # Q14247
Met1-Gly85
Accession # Q14247
Specificity
Detects human and rat Cortactin in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Rat Cortactin Antibody
Detection of Human and Rat Cortactin by Western Blot.
Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, MDA-MB-453 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Cortactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6096) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Cortactin at approximately 80-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human Cortactin Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cortactin knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Cortactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6096) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cortactin at approximately 80 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Rat Cortactin Antibody
Application
Recommended Usage
Knockout Validated
Cortactin
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
Cortactin knockout HeLa cell line.
Western Blot
1 µg/mL
Sample: NTera‑2 human testicular embryonic carcinoma cell line, MDA‑MB‑453 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and NRK rat normal kidney cell line
Sample: NTera‑2 human testicular embryonic carcinoma cell line, MDA‑MB‑453 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and NRK rat normal kidney cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cortactin
Cortactin (also EMS1 and Amplaxin) is an 80‑85 kDa multidomain scaffold-associated protein. It is an actin-binding cytoplasmic protein found at intercellular junctions and near lamellipodia. It is also found in tumor invadopodia, a somewhat analogous structure to lamellipodia. Here, Cortactin appears to coordinate actin assembly with MMP secretion, thus facilitating tumor invasiveness. Human Cortactin is 550 amino acids (aa) in length and contains six-plus Cortactin regions (aa 80‑324) that regulate actin polymerization, and one SH3 domain (aa 496‑548) that binds to N-WASP. Cortactin is highly phosphorylated on Tyr and Ser/Thr, and undergoes acetylation.
Alternate Names
Amplaxin, CTTN, EMS1
Gene Symbol
CTTN
UniProt
Additional Cortactin Products
Product Documents for Human/Rat Cortactin Antibody
Product Specific Notices for Human/Rat Cortactin Antibody
For research use only
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