Human/Rat DYRK2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5408
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 599542
Product Specifications
Immunogen
E. coli-derived recombinant human DYRK2
aa 1-107
Accession # NP_003574
aa 1-107
Accession # NP_003574
Specificity
Detects human and rat DYRK2 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Rat DYRK2 Antibody
Detection of Human and Rat DYRK2 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, CCD-1070Sk human foreskin fibroblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat DYRK2 Monoclonal Antibody (Catalog # MAB5408) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DYRK2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Rat DYRK2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded testis
Sample: Immersion fixed paraffin-embedded testis
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, CCD‑1070Sk human foreskin fibroblast cell line, and L6 rat myoblast cell line
Sample: HepG2 human hepatocellular carcinoma cell line, CCD‑1070Sk human foreskin fibroblast cell line, and L6 rat myoblast cell line
Reviewed Applications
Read 1 review rated 5 using MAB5408 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DYRK2
DYRK2 (Dual-specificity tyrosine [Y] phosphorylation regulated kinase 2) is a 58‑62 kDa member of the MNB/DYRK subfamily, CMGC Ser/Thr protein kinase family of enzymes. It is expressed in testis and shows dual substrate specificity; autophosphorylation on Tyr382 to self‑activate, and a Ser/Thr phosphorylation of target molecules. Substrates include NFATc, glycogen synthase, and p53. p53 phosphorylation on Ser46 initiates cell apoptosis. Human DYRK2 is 601 amino acids (aa) in length. It contains one kinase catalytic domain (aa 222‑535). One isoform variant shows an alternate start site at Met74. This shorter isoform was used for immunization. Thus, over aa 1‑107 (or aa 74‑180 of the long form), human DYRK2 shares 93% aa identity with mouse DYRK2.
Long Name
Dual-specificity Tyrosine-[Y]-phosphorylation Regulated Kinase 2
Alternate Names
dual specificity tyrosine-phosphorylation-regulated kinase 2, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2, EC 2.7.12, EC 2.7.12.1, FLJ21217, FLJ21365
Gene Symbol
DYRK2
UniProt
Additional DYRK2 Products
Product Documents for Human/Rat DYRK2 Antibody
Product Specific Notices for Human/Rat DYRK2 Antibody
For research use only
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