Human/Rat Histone Deacetylase 2/HDAC2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7679
Key Product Details
Species Reactivity
Human, Rat
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 806116
Product Specifications
Immunogen
E. coli-derived recombinant human Histone Deacetylase 2/HDAC-2
Pro386-Pro488
Accession # Q92769
Pro386-Pro488
Accession # Q92769
Specificity
Detects human Histone Deacetylase 2/HDAC-2 in direct ELISAs and human and rat Histone Deacetylase 2/HDAC-2 Western blots. In direct ELISAs, no cross-reactivity with recombinant human HDAC1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Rat Histone Deacetylase 2/HDAC2 Antibody
Detection of Human and Rat Histone Deacetylase 2/HDAC2 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, SH-SY5Y human neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Histone Deacetylase 2/HDAC2 Monoclonal Antibody (Catalog # MAB7679) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Histone Deacetylase 2/HDAC2 by Simple WesternTM.
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 98 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Rat Histone Deacetylase 2/HDAC2 Monoclonal Antibody (Catalog # MAB7679). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Rat Histone Deacetylase 2/HDAC2 Antibody
Application
Recommended Usage
Simple Western
2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Western Blot
0.2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, SH‑SY5Y human neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line
Sample: K562 human chronic myelogenous leukemia cell line, SH‑SY5Y human neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB7679 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Histone Deacetylase 2/HDAC2
Long Name
Histone Deacetylase 2
Alternate Names
HDAC2, YAF1
Gene Symbol
HDAC2
UniProt
Additional Histone Deacetylase 2/HDAC2 Products
Product Documents for Human/Rat Histone Deacetylase 2/HDAC2 Antibody
Product Specific Notices for Human/Rat Histone Deacetylase 2/HDAC2 Antibody
For research use only
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