Human/Rat IkB-beta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3425
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 347428
Product Specifications
Immunogen
E. coli-derived recombinant human IkB-beta
Ala2-Val356
Accession # Q15653
Ala2-Val356
Accession # Q15653
Specificity
Detects human and rat IkB-beta in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IkB-alpha or rhIkB-epsilon is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Rat IkB-beta Antibody
Detection of Human IkB‑ beta by Western Blot.
Western blot shows recombinant human Ik beta-a, IkB-beta, and IkB-e (20 ng/lane). PVDF mem-brane was probed with 1 µg/mL Mouse Anti-Human/Rat IkB-beta Mono-clonal Antibody (Catalog # MAB3425) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Human/Rat IkB-beta was detected at approximately 48-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of Human/Rat IkB‑ beta by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Rat IkB-beta Monoclonal Antibody (Catalog # MAB3425) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific band for Human/Rat IkB-beta were detected at approximately 48 kDa and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human/Rat IkB-beta Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: MCF-7 human breast cancer cell line and PC-12 rat adrenal pheochromocytoma cell line
Sample: MCF-7 human breast cancer cell line and PC-12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IkB-beta
NF kappaB inhibitor beta (IkB-beta) inhibits the NF kappaB complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-beta is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-beta degradation, the NF kappaB complex is able to translocate to the nucleus and activate transcription.
Long Name
I-kappa-B-beta
Alternate Names
IkBbeta, NFKBIB, TR-interacting protein 9
Gene Symbol
NFKBIB
UniProt
Additional IkB-beta Products
Product Documents for Human/Rat IkB-beta Antibody
Product Specific Notices for Human/Rat IkB-beta Antibody
For research use only
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