Human/Rat PAK7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4696
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 444703
Product Specifications
Immunogen
E. coli-derived recombinant human PAK7
Met1-Glu134
Accession # Q9P286
Met1-Glu134
Accession # Q9P286
Specificity
Detects human and rat PAK7 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Rat PAK7 Antibody
Detection of Human/Rat PAK7 by Western Blot.
Western blot shows lysates of human brain tissue, SH-SY5Y human neuroblastoma cell line, Raji human Burkitt's lymphoma cell line, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat PAK7 Monoclonal Antibody (Catalog # MAB4696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PAK7 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.PAK7 in Human Brain.
PAK7 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 25 µg/mL Mouse Anti-Human/Rat PAK7 Monoclonal Antibody (Catalog # MAB4696) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to Purkinje cell bodies and dendrites. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Rat PAK7 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Western Blot
1 µg/mL
Sample: Human brain tissue, SH-SY5Y human neuroblastoma cell line, Raji human Burkitt's lymphoma cell line, and rat brain tissue
Sample: Human brain tissue, SH-SY5Y human neuroblastoma cell line, Raji human Burkitt's lymphoma cell line, and rat brain tissue
Reviewed Applications
Read 1 review rated 5 using MAB4696 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PAK7
Long Name
p21/Cdc42/Rac1-activated Kinase 7
Alternate Names
PAK5
Gene Symbol
PAK5
UniProt
Additional PAK7 Products
Product Documents for Human/Rat PAK7 Antibody
Product Specific Notices for Human/Rat PAK7 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...