Skip to main content

Human/Rat PAK7 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4696

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
MAB4696
MAB4696-SP

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 444703

Product Specifications

Immunogen

E. coli-derived recombinant human PAK7
Met1-Glu134
Accession # Q9P286

Specificity

Detects human and rat PAK7 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Rat PAK7 Antibody

Detection of Human/Rat PAK7 antibody by Western Blot.

Detection of Human/Rat PAK7 by Western Blot.

Western blot shows lysates of human brain tissue, SH-SY5Y human neuroblastoma cell line, Raji human Burkitt's lymphoma cell line, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat PAK7 Monoclonal Antibody (Catalog # MAB4696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PAK7 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
PAK7 antibody in Human Brain by Immunohistochemistry (IHC-P).

PAK7 in Human Brain.

PAK7 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 25 µg/mL Mouse Anti-Human/Rat PAK7 Monoclonal Antibody (Catalog # MAB4696) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to Purkinje cell bodies and dendrites. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Rat PAK7 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Western Blot

1 µg/mL
Sample: Human brain tissue, SH-SY5Y human neuroblastoma cell line, Raji human Burkitt's lymphoma cell line, and rat brain tissue

Reviewed Applications

Read 1 review rated 5 using MAB4696 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PAK7

PAK7 (p21-activated kinase 7), also called PAK5, is a neuronal member of the PAK-II subfamily within the STE Ser/Thr protein kinase family. In the portion used as immunogen, human PAK7 shares 95% aa sequence identity with rat PAK7. This portion contains a CDC42/Rac1 interactive binding (CRIB) motif and potential phosphorylation sites at Ser104 and Thr107. In addition, PAK7 also interacts with RhoD and RhoH, directing its subcellular localization. PAK7 can be located in the mitochondrion, where it protects cells from apoptosis, or in the nucleus.

Long Name

p21/Cdc42/Rac1-activated Kinase 7

Alternate Names

PAK5

Entrez Gene IDs

57144 (Human); 241656 (Mouse); 311450 (Rat)

Gene Symbol

PAK5

UniProt

Additional PAK7 Products

Product Documents for Human/Rat PAK7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Rat PAK7 Antibody

For research use only

Loading...
Loading...
Loading...
Loading...