Human/Rat PIWIL2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6558
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Primate - Pan paniscus (Bonobo), Primate - Pan troglodytes (Chimpanzee)
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PIWIL2
Lys216-Asp379
Accession # Q8TC59
Lys216-Asp379
Accession # Q8TC59
Specificity
Detects human PIWIL2 in direct ELISAs and human and rat PIWIL2 in Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human PIWIL4 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Rat PIWIL2 Antibody
Detection of Rat PIWIL2 by Western Blot.
Western blot shows lysates of rat testis tissue. PVDF Membrane was probed with 1 µg/mL of Human PIWIL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6558) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PIWIL2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human/Rat PIWIL2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Rat testis tissue
Sample: Rat testis tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PIWIL2
PIWIL2 (PIWI [P-element-induced wimpy testis]-like 2; also HILI) is a 100-110 kDa cytosolic member of the PIWI subfamily, Argonaute family of proteins. It is expressed in the germ line, particularly in premeiotic and early meiotic germ cells, plus mesenchymal stem cells, and in select cancer stem cells such as CD44+CD24- breast cancer cells. PIWIL2 likely silences mobile genetic elements in germ cells, and appears to depress stem cell proliferation by inhibiting the expression of cell cycle genes. Human PIWIL2 is 973 amino acids (aa) in length. It contains one PAZ domain (aa 384-496) and a C-terminal PIWI domain (aa 668-959) that interact with RNA. There are multiple methylation sites on arginines. Two potential splice forms are reported, one that shows a deletion of aa 887-922, and a second that contains a 12 aa substitution for aa 486-801. Over aa 216-379, human PIWIL2 shares 96% aa identity with rat PIWIL2.
Long Name
Piwi-like Protein 2
Alternate Names
CT80, HILI, MILI
Gene Symbol
PIWIL2
UniProt
Additional PIWIL2 Products
Product Documents for Human/Rat PIWIL2 Antibody
Product Specific Notices for Human/Rat PIWIL2 Antibody
For research use only
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