Human/Rat PKC beta1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3759

R&D Systems, part of Bio-Techne
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AF3759
AF3759-SP
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all PKC beta 1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKC beta1
Lys607-Val671
Accession # P05771

Specificity

Detects human and rat PKC beta1 in direct ELISAs. In direct ELISAs, less than 1% cross‑reactivity with recombinant human PKC delta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Rat PKC beta1 Antibody

PKC beta1 antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

PKC beta1 in Rat Cortical Stem Cells.

PKC beta1 was detected in immersion fixed rat cortical stem cells using Goat Anti-Human/Rat PKC beta1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; Catalog # NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Rat PKC beta1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human neural progenitor cells and immersion fixed rat cortical stem cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human PKC beta1
Recombinant Rat PKC beta1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC beta 1

PKC beta1 is a 671 aa, 77 kDa member of the PKC subfamily of Ser/Thr family of protein kinases. PKC beta1 is a Ca2+-activated, phospholipid-dependent enzyme responsible for the phosphorylation of multiple proteins. Human PKC beta2 is an alternatively spliced isoform with a 52 aa substitution of the 50 C-terminal residues. In monkey, five alternativly spliced forms have been reported. Over the region used as immunogen, human and rat PKC beta1 are 100% identical.

Long Name

Protein Kinase C beta 1

Alternate Names

PRKCB, PRKCB1, PRKCB2

Entrez Gene IDs

5579 (Human); 18751 (Mouse); 25023 (Rat)

Gene Symbol

PRKCB

UniProt

P05771

Additional PKC beta 1 Products

Product Documents for Human/Rat PKC beta1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Rat PKC beta1 Antibody

For research use only

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