Human/Rat PTK7/CCK4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4499
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 525222
Product Specifications
Immunogen
E. coli-derived recombinant human PTK7/CCK4
Ala31-Ser199
Accession # Q13308
Ala31-Ser199
Accession # Q13308
Specificity
Detects human and rat PTK7/CCK4 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Rat PTK7/CCK4 Antibody
Detection of Human and Rat PTK7/CCK4 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat PTK7/CCK4 Monoclonal Antibody (Catalog # MAB4499) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTK7/CCK4 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PTK7/CCK4 in Human Colon Cancer Tissue.
PTK7/CCK4 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human/Rat PTK7/CCK4 Monoclonal Antibody (Catalog # MAB4499) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human PTK7/CCK4 by Simple WesternTM.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTK7/CCK4 at approximately 168 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Rat PTK7/CCK4 Monoclonal Antibody (Catalog # MAB4499). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Applications for Human/Rat PTK7/CCK4 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue
Simple Western
20 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and Rat-2 rat embryonic fibroblast cell line
Sample: HepG2 human hepatocellular carcinoma cell line and Rat-2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTK7/CCK4
Long Name
Protein Tyrosine Kinase 7
Alternate Names
CCK4
Gene Symbol
PTK7
UniProt
Additional PTK7/CCK4 Products
Product Documents for Human/Rat PTK7/CCK4 Antibody
Product Specific Notices for Human/Rat PTK7/CCK4 Antibody
For research use only
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