Human/Rat Yes Antibody

Name: Human/Rat Yes Antibody
Brand: R&D Systems
SKU: AF3205

R&D Systems, part of Bio-Techne | Catalog # AF3205

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Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Yes Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Yes
Met1-Gly88
Accession # P07947.3

Specificity

Detects human and rat Yes.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Rat Yes Antibody

Detection of Human/Rat Yes by Western Blot.

Western blot shows lysates of L6 rat myoblast cell line, Rat-2 rat embryonic fibroblast cell line, MCF-7 human breast cancer cell line and, MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Rat Yes Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3205) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Yes at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Yes in A431 Human Cell Line.

Yes was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human/Rat Yes Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3205) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Rat Yes Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line

Western Blot

0.5 µg/mL
Sample: L6 rat myoblast cell line, Rat-2 rat embryonic fibroblast cell line, MCF-7 human breast cancer cell line and, MDA-MB-468 human breast cancer cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Yes

Yes is the cellular homolog of the oncogenic protein encoded by the Yamaguchi 73 and Esh avian sarcoma viruses. After Src, Yes is the most widely expressed member of the Src family of nonreceptor tyrosine kinases. Src family kinases regulate an array of cellular processes, including growth factor signaling, cytoskeleton dynamics, and cell proliferation.

Long Name

V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1

Alternate Names

YES1

Entrez Gene IDs

7525 (Human); 22612 (Mouse); 24884 (Rat)

Gene Symbol