Detects human Ret in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse Ret is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Ret APC-conjugated Antibody

Detection of Ret in SH‑SY5Y Human Cell Line by Flow Cytometry.

SH-SY5Y human neuroblastoma cell line was stained with Mouse Anti-Human Ret APC-conjugated Monoclonal Antibody (Catalog # IC718A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Ret APC-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: SH‑SY5Y human neuroblastoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Ret

The GDNF family of neurotrophic factors forms a subfamily within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and the adult animal. The GDNF family members (GDNF, neurturin, artemin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha-1‑4) (1). GFR alpha-1 -2, -3 and -4 are the preferred ligand-binding subunits for GDNF, neurturin, artemin and persephin, respectively. The Ret tyrosine-kinase receptor is a 170-175 kDa type I transmembrane glycoprotein that is encoded by the c-ret proto-oncogene (2,3). Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma (4). Human and mouse Ret share 83% amino acid (aa) sequence identity (77% identity in the extracellular domain over aa 29-635). Although Ret does not bind GDNF ligands directly, in general, the extracellular domain of Ret binds the GDNF-GFR-alpha complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha (5-6).

References

Wang, X. (2013) Biochim. Biophys. Acta. 1834:2205.
Takahashi, M. and G.M. Cooper (1987) Mol. Cell Biol. 7:1378.
Richardson, D.S. et al. (2012) Mol. Cell Biol. 23:3838.
Mulligan, L.M. et al. (2014) Nat. Rev. Cancer 14:173.
Ibanez, C.F. (2013) Cold Spring Harb. Perspect. Biol. 5:a009134.
Plaza-Menacho, I. et al. (2014) Cell. Signal. 26:1743.

Long Name

Proto-oncogene Tyrosine-protein Kinase Receptor Ret

Alternate Names

CDHF12MTC1, CDHR16, EC 2.7.10, Hirschsprung disease 1, Hirschsprung disease), MEN2A, MEN2B, multiple endocrine neoplasia and medullary thyroid carcinoma 1, ret proto-oncogene, RET transforming sequence

Entrez Gene IDs

5979 (Human); 19713 (Mouse)

Gene Symbol

RET

UniProt

P07949

Additional Ret Products

Product Documents for Human Ret APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Ret APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human Ret APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC718A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications