Human RFX6 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7780

R&D Systems, part of Bio-Techne
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AF7780
AF7780-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all RFX6 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human RFX6
Lys324-Thr511
Accession # Q8HWS3

Specificity

Detects human RFX6 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human RFX6 Antibody

RFX6 antibody in Human Pancreas by Immunohistochemistry (IHC-P).

RFX6 in Human Pancreas.

RFX6 was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human RFX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7780) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human RFX6 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RFX6

RFX6 (regulatory factor X, member 6) is a 928 amino acid, ~102 kDa (calculated) member of the RFX family of transcription factors that are critical for development. RFX6 is expressed in the gut endoderm, later becoming restricted to developing and adult pancreatic islets. It is downstream of Neurogenin-3 and essential for differentiation of most islet cell types. Mutation in humans can produce autosomal recessive neonatal diabetes. Human RFX6 shares 96% and 95% amino acid sequence identity with mouse and rat RFX6, respectively.

Long Name

Regulatory Factor X, 6

Alternate Names

MTFS, RFXDC1

Entrez Gene IDs

222546 (Human); 320995 (Mouse); 294395 (Rat)

Gene Symbol

RFX6

UniProt

Q8HWS3

Additional RFX6 Products

Product Documents for Human RFX6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human RFX6 Antibody

For research use only

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