Human RIPK3/RIP3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB76042
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1312D
Product Specifications
Immunogen
Synthetic peptide containing human RIPK3/RIP3
Accession # Q9Y572
Accession # Q9Y572
Specificity
Detects human RIPK3/RIP3 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human RIPK3/RIP3 Antibody
Detection of Human RIPK3/RIP3 by Western Blot.
Western blot shows lysates of SK-BR-3 human breast cancer cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB76042) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for RIPK3/RIP3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RIPK3/RIP3 in SK-BR-3 and HepG2 Human Cell Lines.
RIPK3/RIP3 was detected in immersion fixed SK-BR-3 human breast cancer cell line (positive stain) and HepG2 human hepatocellular carcinoma cell line (negative stain) using Rabbit Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB76042) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human RIPK3/RIP3 Antibody
Application
Recommended Usage
Immunocytochemistry
1-25 µg/mL
Sample: Immersion fixed SK-BR-3 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line
Sample: Immersion fixed SK-BR-3 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line
Western Blot
2 µg/mL
Sample: SK‑BR‑3 human breast cancer cell line and Raji human Burkitt's lymphoma cell line
Sample: SK‑BR‑3 human breast cancer cell line and Raji human Burkitt's lymphoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB76042 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RIPK3/RIP3
Long Name
Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 3
Alternate Names
RIP3
Gene Symbol
RIPK3
UniProt
Additional RIPK3/RIP3 Products
Product Documents for Human RIPK3/RIP3 Antibody
Product Specific Notices for Human RIPK3/RIP3 Antibody
For research use only
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