Human RIPK3/RIP3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7604
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Porcine
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Co-Immunoprecipitation, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 780115
Product Specifications
Immunogen
E. coli-derived recombinant human RIPK3/RIP3
Met1-Arg218
Accession # Q9Y572
Met1-Arg218
Accession # Q9Y572
Specificity
Detects human RIPK3/RIP3 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human RIPK3/RIP3 Antibody
Detection of Human RIPK3/RIP3 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB7604) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RIPK3/RIP3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RIPK3/RIP3 in Human Spleen.
RIPK3/RIP3 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB7604) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human RIPK3/RIP3 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen
Sample: Immersion fixed paraffin-embedded sections of human spleen
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and HT‑29 human colon adenocarcinoma cell line
Sample: K562 human chronic myelogenous leukemia cell line and HT‑29 human colon adenocarcinoma cell line
Reviewed Applications
Read 2 reviews rated 5 using MAB7604 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RIPK3/RIP3
Long Name
Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 3
Alternate Names
RIP3
Gene Symbol
RIPK3
UniProt
Additional RIPK3/RIP3 Products
Product Documents for Human RIPK3/RIP3 Antibody
Product Specific Notices for Human RIPK3/RIP3 Antibody
For research use only
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