Human RLK/TXK Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6520
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 674801
Product Specifications
Immunogen
E.coli-derived recombinant human RLK/TXK
Trp150-Val246
Accession # P42681
Trp150-Val246
Accession # P42681
Specificity
Detects human RLK/TXK in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human RLK/TXK Antibody
Detection of Human RLK/TXK by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human primary differentiated Th2 cells. PVDF Membrane was probed with 1 µg/mL of Human RLK/TXK Monoclonal Antibody (Catalog # MAB6520) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for RLK/TXK at approximately 58 and 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human RLK/TXK Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and human primary differentiated Th2 cells
Sample: Jurkat human acute T cell leukemia cell line and human primary differentiated Th2 cells
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RLK/TXK
RLK (resting lymphocyte kinase), also called TXK (T cell-expressed kinase) or PTK4, is a protein tyrosine kinase of the TEK family. It is expressed in T cells and some myeloid cells, especially in Th1 cells in inflammatory diseases such as rheumatoid arthritis and Behcet’s disease. Upon TCR stimulation, it translocates to the nucleus and induces IFN-gamma production. An alternate start site creates a resident nuclear 55 kDa form in addition to the larger (~58 kDa) form that is cytoplasmic or targeted to the plasma membrane via palmitoylation of a cysteine string motif. The region used as an immunogen is common to both forms and shares 79% and 81% amino acid identity with mouse and rat RLK, respectively.
Long Name
Resting Lymphocyte Kinase
Alternate Names
BTKL, PSCTK5, PTK4, TXK
Entrez Gene IDs
7294 (Human)
Gene Symbol
TXK
UniProt
Additional RLK/TXK Products
Product Documents for Human RLK/TXK Antibody
Product Specific Notices for Human RLK/TXK Antibody
For research use only
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