Human RUNX3/CBFA3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3765
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human RUNX3/CBFA3
Lys186-Tyr415
Accession # Q13761
Lys186-Tyr415
Accession # Q13761
Specificity
Detects human RUNX3/CBFA3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows less than 1% cross‑reactivity with recombinant human (rh) CBFA1 and rhCBFA2.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human RUNX3/CBFA3 Antibody
RUNX3/CBFA3 in Raji Human Cell Line.
RUNX3/CBFA3 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Goat Anti-Human RUNX3/CBFA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3765) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human RUNX3/CBFA3 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human RUNX3/CBFA3
Sample: Recombinant Human RUNX3/CBFA3
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RUNX3/CBFA3
Long Name
Runt-related Transcription Factor 3
Alternate Names
AML2, CBFA3, PEBP2A3, PEBP2AC
Gene Symbol
RUNX3
UniProt
Additional RUNX3/CBFA3 Products
Product Documents for Human RUNX3/CBFA3 Antibody
Product Specific Notices for Human RUNX3/CBFA3 Antibody
For research use only
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