Human S100A6 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7467
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 754102
Product Specifications
Immunogen
E. coli-derived recombinant human S100A6
Met1-Gly90
Accession # P06703
Met1-Gly90
Accession # P06703
Specificity
Detects human S100A6 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity
with recombinant mouse S100A6 and no cross-reactivity with recombinant human
(rh) S100A1, rhS100A4, or rhS100B is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human S100A6 Antibody
Detection of Human and Mouse S100A6 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human S100A6 Monoclonal Antibody (Catalog # MAB7467) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for S100A6 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
S100A6 in Human Kidney.
S100A6 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human S100A6 Monoclonal Antibody (Catalog # MAB7467) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to glomeruli and distal tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human S100A6 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Western Blot
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Reviewed Applications
Read 1 review rated 5 using MAB7467 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A6
Long Name
S100 Calcium Binding Protein A6
Alternate Names
CACY, MLN 4, PRA
Gene Symbol
S100A6
UniProt
Additional S100A6 Products
Product Documents for Human S100A6 Antibody
Product Specific Notices for Human S100A6 Antibody
For research use only
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