Human S100B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB18201
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 721703
Product Specifications
Immunogen
E. coli-derived recombinant human S100B
Lys1-Glu92
Accession # P04271
Lys1-Glu92
Accession # P04271
Specificity
Detects human S100B in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human S100B Antibody
Human S100B ELISA Standard Curve.
Recombinant Human S100B protein was serially diluted 2-fold and captured by Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB18201) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB18202) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human S100B Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB18202).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human S100B DuoSet ELISA Kit (Catalog # DY1820-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100B
Long Name
S100 Calcium Binding Protein B
Alternate Names
beta (neural), NEF, S100, S100 beta, S100 calcium binding protein B, S100 calcium-binding protein B, S100 calcium-binding protein, beta (neural), S-100 calcium-binding protein, beta chain, 10protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100beta
Entrez Gene IDs
6285 (Human)
Gene Symbol
S100B
UniProt
Additional S100B Products
Product Documents for Human S100B Antibody
Product Specific Notices for Human S100B Antibody
For research use only
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