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Human S1P1/EDG-1 Alexa Fluor® 700-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2016N

R&D Systems, part of Bio-Techne
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FAB2016N

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 218713

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human S1P1/EDG‑1
Met1-Ser382
Accession # P21453

Specificity

Detects human S1P1/EDG‑1 by flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human S1P1/EDG-1 Alexa Fluor® 700-conjugated Antibody

Detection of S1P1/EDG-1 antibody in CHO Chinese Hamster Cell Line Transfected with Human S1P1/EDG-1 and eGFP antibody by Flow Cytometry.

Detection of S1P1/EDG‑1 in CHO Chinese Hamster Cell Line Transfected with Human S1P1/EDG-1 and eGFP by Flow Cytometry.

CHO Chinese hamster ovary cell line transfected with either (A) human S1P1/EDG-1 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human S1P1/EDG-1 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB2016N). Quadrant markers were set based on control antibody staining (Catalog # IC0041N). View our protocol for Staining Membrane-associated Proteins.

Applications for Human S1P1/EDG-1 Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: CHO Chinese hamster ovary cell line transfected with S1P1/EDG-1 and eGFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P1/EDG-1

S1P1, also known as EDG-1, is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. S1P1 signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis, and angiogenesis.

Long Name

Endothelial Differentiation Gene 1

Alternate Names

CD363, EDG1, S1PR1

Entrez Gene IDs

1901 (Human); 13609 (Mouse)

Gene Symbol

S1PR1

UniProt

Additional S1P1/EDG-1 Products

Product Documents for Human S1P1/EDG-1 Alexa Fluor® 700-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human S1P1/EDG-1 Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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