Human S1P1/EDG-1 Antibody

Detection of S1P₁/EDG‑1 in CHO Chinese Hamster Cell Line Transfected with Human S1P₁/EDG-1 and eGFP by Flow Cytometry.

CHO Chinese hamster ovary cell line transfected with either (A) human S1P₁/EDG-1 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human S1P₁/EDG-1 Monoclonal Antibody (Catalog # MAB2016) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Detection of Human S1P1/EDG-1 by Flow Cytometry

S1PR1 expression by OECs is dynamically altered by microenvironment.OEC expression of S1PR1 under normoxia and hypoxia was qualitatively confirmed by immunocytochemistry (A). The percentage of cells expressing S1PR1 on the cell surface, as confirmed with flow cytometry, was enhanced under hypoxia as compared to the normoxic control (B). Combined stimuli of S1P and VEGF resulted in greater S1PR1 mRNA production after 24h in normoxia (C). Combined stimuli also resulted in a greater proportion of S1PR1+ OECs under both normoxia and hypoxia (D and E). Scale bars represent 40 μm. Data are mean ± SD (n = 4) and normalized to the average untreated control values for either normoxia or hypoxia (C, E; indicated by dashed line) accordingly. An asterisk indicates statistically significant differences (P<0.05) between conditions in normoxia or hypoxia respectively and N.S. displays no statistically significant difference between conditions. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0123437), licensed under a CC-BY license. Not internally tested by R&D Systems.