Human S1P₃/EDG-3 and eGFP co-transfectants were stained with either (A) Mouse IgG2A Isotype Control (Catalog # MAB003) or (B) Mouse Anti-Human S1P₃/EDG-3 Monoclonal Antibody (Catalog # MAB7524) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human S1P3/EDG-3 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human S1P₃/EDG-3 and eGFP co-transfectants

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S1P3/EDG-3

Sphingosine 1-Phosphate receptor-3 (S1P3), also known as EDG-3, is a 378 amino acid (aa) seven-transmembrane receptor for the lysolipid phosphoric acid mediator, sphingosine 1-phosphate (S1P). S1P3 interactions with S1P induce a range of responses including vasoconstriction, brachycardia, vascular endothelial and smooth muscle cell proliferation and migration, heart and liver fibrosis, and coagulation-induced inflammation. Extracellular portions of human S1P3 show 74% aa identity with mouse and rat S1P3.

Long Name

Endothelial Differentiation Gene 3

Alternate Names

EDG3, LPB3, S1PR3

Entrez Gene IDs

1903 (Human); 13610 (Mouse); 306792 (Rat)

Gene Symbol

S1PR3

UniProt

Q99500 (Human)

Additional S1P3/EDG-3 Products

Product Documents for Human S1P3/EDG-3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human S1P3/EDG-3 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human S1P3/EDG-3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7524

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all S1P3/EDG-3 Antibodies »

Product Summary for Human S1P3/EDG-3 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human S1P3/EDG-3 Antibody

Detection of S1P3/EDG-3 in S1P3/EDG-3 and eGFP Co-Transfected Cells by Flow Cytometry.