Human SALM2/LRFN1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5669

R&D Systems, part of Bio-Techne
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AF5669
AF5669-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all SALM2/LRFN1 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human SALM2/LRFN1

Specificity

Detects human SALM2/LRFN1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) SALM3 and rhSALM4 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human SALM2/LRFN1 Antibody

Detection of Human SALM2 antibody by Western Blot.

Detection of Human SALM2 by Western Blot.

Western blot shows lysates of human brain cortex and hipoocampus tissue and negative control lung and liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SALM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5669) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for SALM2 at approximately 90-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human SALM2/LRFN1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human brain cortex and hipoocampus tissue and negative control lung and liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SALM2/LRFN1

Synaptic adhesion-like molecule 2 (SALM2; also leucine-rich repeat and fibronectin type-III domain-containing protein 1 (Lrfn1) is a 105 kDa member of the Lrfn family of type I transmembrane glycoproteins (1‑2). Human SALM2 is synthesized as a 771 amino acid (aa) precursor that contains a 31 aa signal sequence, a 505 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 214 aa cytoplasmic region.  The ECD consists of seven leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order (1, 3). In addition, there are two potential sites for N-linked glycosylation. The C-terminal region contains an intracellular PDZ binding domain, which is conserved among SALMs 1‑3, but is absent in SALMs 4 and 5 (4). Mature human SALM2 shares 96% and 95% aa sequence identity with mature mouse and rat SALM2, respectively. In the developing mouse embryo, the temporal expression profile blot revealed a general increment of expression around E10.5 for SALM2. Northern blot analysis showed that in rats, SALM2 is strongly expressed in the adult brain and is also present in the adult testis (2). SALM2 localizes to both axons and dendrites (4). In addition, it co-localizes with both pre- and post-synaptic proteins at excitatory synapses in mature neurons (2, 4). SALM2, like the other SALMs, promotes neurite outgrowth (4). In particular, SALM2 promotes an increase in the number of primary processes compared to controls and SALMs 1 and 5 (4). In addition, SALM2 is an important regulator of the differentiation of excitatory synapses (2). SALM2 may also be involved in synaptic maintenance and other cellular interactions (4).

References

  1. Morimura, N. et al. (2006) Gene 380:72.
  2. Ko, J. et al. (2006) Neuron 50:233.
  3. Wang, C.-Y. et al. (2006) J. Neurosci. 26:2174.
  4. Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.

Long Name

Synaptic Adhesion-like Molecule 2/Leucine-rich Repeat and Fibronectin Type III Domain Containing 1

Alternate Names

LRFN1

Entrez Gene IDs

57622 (Human); 365222 (Rat)

Gene Symbol

LRFN1

Additional SALM2/LRFN1 Products

Product Documents for Human SALM2/LRFN1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human SALM2/LRFN1 Antibody

For research use only

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