Human SALM4/LRFN3 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5349

R&D Systems, part of Bio-Techne
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AF5349
AF5349-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all SALM4/LRFN3 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SALM4/LRFN3
Ser17-Met539
Accession # Q9BTN0

Specificity

Detects human SALM4/LRFN3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human SALM3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SALM4/LRFN3 Antibody

Detection of Human SALM4/LRFN3 antibody by Western Blot.

Detection of Human SALM4/LRFN3 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SALM4/LRFN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5349) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SALM4/LRFN3 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human SALM4/LRFN3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SALM4/LRFN3

Synaptic adhesion-like molecule 4 (SALM4; also leucine-rich repeat and fibronectin type-III domain-containing protein 3 (Lrfn3) is an approximately 90 kDa member of the Lrfn family of type I transmembrane glycoproteins (1). Human SALM4 is synthesized as a 628 amino acid (aa) precursor that contains a 16 aa signal sequence, a 523 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 68 aa cytoplasmic region. The ECD consists of six leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order (1, 2). In addition, there are five potential sites for N-linked glycosylation. SALM4 and -5 lack a C-terminal intracellular PDZ binding domain, which is conserved among SALMs 1‑3. Mature human SALM4 shares 96% aa sequence identity with mature mouse SALM4. Northern blot analysis showed that in mice, SALM4 is strongly expressed in the adult brain and is also present in the adult gastrointestinal tract and kidneys (1). It is distributed throughout the neuron, including the growth cone (3). In the developing mouse embryo, a temporal expression profile blot revealed a general increment of expression around E10.5, with weak expression detected before E10.5 (1). SALM4, like the other SALMs, promotes neurite outgrowth (3). Specifically, the SALMs modify total outgrowth and neurite branching (3).

References

  1. Morimura, N. et al. (2006) Gene 380:72.
  2. Wang, C.-Y. et al. (2006) J. Neurosci. 26:2174.
  3. Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.

Long Name

Leucine-rich Repeat and Fibronectin Type III Domain Containing Protein 3

Alternate Names

FIGLER1, LRFN3

Entrez Gene IDs

79414 (Human); 233067 (Mouse); 308495 (Rat)

Gene Symbol

LRFN3

UniProt

Q9BTN0

Additional SALM4/LRFN3 Products

Product Documents for Human SALM4/LRFN3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human SALM4/LRFN3 Antibody

For research use only

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