Human SEC13 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF9055
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Simple Western, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SEC13
Gly123-Gln322
Accession # P55735
Gly123-Gln322
Accession # P55735
Specificity
Detects human SEC13 in direct ELISA and Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human SEC13 Antibody
Detection of Human SEC13 by Western Blot.
Western blot shows lysates of human kidney tissue and human lung tissue. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human SEC13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9055) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SEC13 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SEC13 in HeLa Human Cell Line.
SEC13 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human SEC13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9055) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human SEC13 by Simple WesternTM.
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for SEC13 at approximately 42 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human SEC13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9055). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human SEC13 Antibody
Application
Recommended Usage
Immunocytochemistry
1-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Simple Western
0.5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Western Blot
0.05 µg/mL
Sample: Human kidney tissue and human lung tissue
Sample: Human kidney tissue and human lung tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SEC13
Long Name
Nuclear Pore Complex Protein Nup85
Alternate Names
D3S1231E, Npp-20, SEC13L1, SEC13R
Gene Symbol
SEC13
UniProt
Additional SEC13 Products
Product Documents for Human SEC13 Antibody
Product Specific Notices for Human SEC13 Antibody
For research use only
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