Human Secretagogin Antibody

Detection of Human and Mouse Secretagogin by Western Blot.

Western blot shows lysates of betaTC-6 mouse beta cell insulinoma cell line and human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Secretagogin Monoclonal Antibody (Catalog # MAB4878) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Secretagogin at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Secretagogin in Human Pancreas.

Secretagogin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Secretagogin Monoclonal Antibody (Catalog # MAB4878) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human and Mouse Secretagogin by Simple Western^TM.

Simple Western lane view shows lysates of betaTC‑6 mouse beta cell insulinoma cell line and human pancreas tissue, loaded at 0.5 mg/mL. A specific band was detected for Secretagogin at approximately 37 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Secretagogin Monoclonal Antibody (Catalog # MAB4878). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.