Human Semaphorin 7A Alexa Fluor® 594-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB20681T

R&D Systems, part of Bio-Techne
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FAB20681T-100UG
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 310829

View all Semaphorin 7A Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 7A
His47-Ala648
Accession # O75326

Specificity

Detects human Semaphorin 7A in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Semaphorin 7A Alexa Fluor® 594-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Human T cells treated with PHA

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Semaphorin 7A

Semaphorin 7A (Sema7A, designated CD108, previously Sema K1 or Sema L), is an ~80 kDa membrane-anchored glycoprotein that is a member of the Semaphorin family of axon guidance molecules (1‑4). On human erythrocytes, it is the John Milton Hagen (JMH) blood group antigen (4). Sema7A is the only known Class 7 or glycophosphatidylinositol (GPI)-linked semaphorin; its expression is concentrated in the brain, spleen and thymus (1‑5). Human Sema7A cDNA encodes a 44 amino acid (aa) signal sequence, a 604 aa extracellular domain (ECD) including Sema and C2‑type Ig-like domains, and an 18 aa propeptide/GPI membrane anchor signal sequence. Mature human Sema7A shares 89%, 89%, 88%, 86% and 90% aa identity with mouse, rat, canine, bovine and equine Sema7A, respectively. The Sema7A sema domain contains an RGD integrin interaction motif (4). Although it binds plexin-C1 in vitro and may be coexpressed with it, many of its activities depend on interaction with beta1 integrins such as alpha1 beta1 (6‑10). Sema7A signaling through the two receptors may cause opposing effects (8). Sema7A is an immune semaphorin, with expression and activity on CD4+CD8+ thymocytes, activated T cells, macrophages and microglia (2, 9‑12). T cell Sema7A interacts with monocytic cells, stimulating their chemotaxis, production of pro-inflammatory cytokines, and dendritic differentiation (5, 6). However, on the T cells themselves, Sema7A downregulates TCR signaling by promoting TCR internalization, modulating T cell responses (9). In lung macrophages, Sema7A is induced by TGF-beta and participates in TGF-beta -induced lung fibrosis (12). Sema7A is also expressed on pre-osteoblasts and osteoclasts, where it promotes migration and fusion, respectively; on keratinocytes, where it promotes melanocyte spreading and dendricity; and on some neurons, for example, promoting axon outgrowth in the developing olfactory tract (8, 10, 13). 

References

  1. Yazdani, U. and J.R. Terman (2006) Genome Biol. 7:211.
  2. Kikutani, H. et al. (2007) Adv. Immunol. 93:121.
  3. Sato, Y. and Takahashi H. (1998) Biochim. Biophys. Acta. 1443:419.
  4. Yamada, A. et al. (1999) J. Immunol. 162:4094.
  5. Holmes, S. et al. (2002) Scand. J. Immunol. 56:270.
  6. Suzuki, K. et al. (2007) Nature 446:680.
  7. Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98.
  8. Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
  9. Czopik, A.K. et al. (2006) Immunity 24:591.
  10. Pasterkamp, R.J. et al. (2003) Nature 424:398.
  11. Mine, T. et al. (2000) Tissue Antigens 55:429.
  12. Kang, H.-R. et al. (2007) J. Exp. Med. 204:1083.
  13. Delorme, G. et al. (2005) Biol. Cell 97:589.

Alternate Names

CD108, H-Sema-L, Sema7A, SEMAL

Entrez Gene IDs

8482 (Human); 20361 (Mouse)

Gene Symbol

SEMA7A

UniProt

O75326

Additional Semaphorin 7A Products

Product Documents for Human Semaphorin 7A Alexa Fluor® 594-conjugated Antibody

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Product Specific Notices for Human Semaphorin 7A Alexa Fluor® 594-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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