Human Semaphorin 7A APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB20681A

R&D Systems, part of Bio-Techne
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FAB20681A
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry, Immunohistochemistry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 310829

View all Semaphorin 7A Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 7A
His47-Ala648
Accession # O75326

Specificity

Detects human Semaphorin 7A in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Semaphorin 7A APC-conjugated Antibody

Detection of Semaphorin 7A antibody in Human PBMCs antibody by Flow Cytometry.

Detection of Semaphorin 7A in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with PHA were stained with Mouse Anti-Human Semaphorin 7A APC-conjugated Monoclonal Antibody (Catalog # FAB20681A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Semaphorin 7A APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PHA

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Semaphorin 7A

Semaphorin 7A (Sema7A, designated CD108, previously Sema K1 or Sema L), is an ~80 kDa membrane-anchored glycoprotein that is a member of the Semaphorin family of axon guidance molecules (1-4). On human erythrocytes, it is the John Milton Hagen (JMH) blood group antigen (4). Sema7A is the only known Class 7 or glycophosphatidylinositol (GPI)-linked semaphorin; its expression is concentrated in the brain, spleen and thymus (1-5). Human Sema7A cDNA encodes a 44 amino acid (aa) signal sequence, a 604 aa extracellular domain (ECD) including Sema and C2-type Ig-like domains, and an 18 aa propeptide/GPI membrane anchor signal sequence. Mature human Sema7A shares 89%, 89%, 88%, 86% and 90% aa identity with mouse, rat, canine, bovine and equine Sema7A, respectively. The Sema7A sema domain contains an RGD integrin interaction motif (4). Although it binds plexin-C1 in vitro and may be coexpressed with it, many of its activities depend on interaction with beta1 integrins such as alpha1 beta1 (6-10). Sema7A signaling through the two receptors may cause opposing effects (8). Sema7A is an immune semaphorin, with expression and activity on CD4+CD8+ thymocytes, activated T cells, macrophages and microglia (2, 9-12). T cell-expressed Sema7A interacts with monocytic cells, stimulating their chemotaxis, production of pro-inflammatory cytokines, and dendritic differentiation (5, 6). However, on the T cells themselves, Sema7A downregulates TCR signaling by promoting TCR internalization, modulating T cell responses (9). In lung macrophages, Sema7A is induced by TGF-beta and participates in TGF-beta -induced lung fibrosis (12). Sema7A is also expressed on pre-osteoblasts and osteoclasts, where it promotes migration and fusion, respectively; on keratinocytes, where it promotes melanocyte spreading and dendricity; and on some neurons, for example, promoting axon outgrowth in the developing olfactory tract (8, 10, 13). 

References

  1. Yazdani, U. and J.R. Terman (2006) Genome Biol. 7:211.
  2. Kikutani, H. et al. (2007) Adv. Immunol. 93:121.
  3. Sato, Y. and Takahashi H. (1998) Biochim. Biophys. Acta. 1443:419.
  4. Yamada, A. et al. (1999) J. Immunol. 162:4094.
  5. Holmes, S. et al. (2002) Scand. J. Immunol. 56:270.
  6. Suzuki, K. et al. (2007) Nature 446:680.
  7. Pasterkamp, R.J. et al. (2007) BMC Dev. Biol. 7:98.
  8. Scott, G.A. et al. (2007) J. Invest. Dermatol. 128:151.
  9. Czopik, A.K. et al. (2006) Immunity 24:591.
  10. Pasterkamp, R.J. et al. (2003) Nature 424:398.
  11. Mine, T. et al. (2000) Tissue Antigens 55:429.
  12. Kang, H.R. et al. (2007) J. Exp. Med. 204:1083.
  13. Delorme, G. et al. (2005) Biol. Cell 97:589.

Alternate Names

CD108, H-Sema-L, Sema7A, SEMAL

Entrez Gene IDs

8482 (Human); 20361 (Mouse)

Gene Symbol

SEMA7A

UniProt

O75326

Additional Semaphorin 7A Products

Product Documents for Human Semaphorin 7A APC-conjugated Antibody

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Product Specific Notices for Human Semaphorin 7A APC-conjugated Antibody

For research use only

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