Human Serpin A5/Protein C Inhibitor Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1266
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A5/Protein C Inhibitor
His20-Pro406
Accession # P05154
His20-Pro406
Accession # P05154
Specificity
Detects human Serpin A5/Protein C in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A4, rhSerpin A9, and rhSerpin A11 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Serpin A5/Protein C Inhibitor Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin A5/Protein C Inhibitor (Catalog # 1266-PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin A5/Protein C Inhibitor (Catalog # 1266-PI), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Serpin A5/Protein C Inhibitor (Catalog # 1266-PI)
Sample: Recombinant Human Serpin A5/Protein C Inhibitor (Catalog # 1266-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A5/Protein C Inhibitor
Serpin A5 is the a member of the Serpin superfamily and inhibits a variety of serine proteases such as protein C, plasminogen activators, thrombin, factor Xa, several kallekreins and acrosin (1). Serpin A5 is synthesized in the liver and secreted in plasma. It is found in numerous steroid-responsive organs and has been detected in saliva, cerebral spinal fluid, amniotic fluid, tears and semen. Because of its protease targets and regulated expression patterns, Serpin A5 has been proposed to play a role in processes such as blood coagulation, fertilization and carcinogenesis (2, 3). Similar to Serpins C1 and D1, its thrombin inhibitory activity is enhanced by heparin.
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Palmier, D. et al. (2002) J. Biol. Chem. 277:40950.
- Geiger, M. et al. (1996) Immunopharmacology 32:53.
Alternate Names
PAI-3, PCI
Gene Symbol
SERPINA5
UniProt
Additional Serpin A5/Protein C Inhibitor Products
Product Specific Notices for Human Serpin A5/Protein C Inhibitor Antibody
For research use only
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