Human Serpin A7/TBG Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8176
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Serpin A7/TBG
Ala21-Ala415
Accession # P05543
Ala21-Ala415
Accession # P05543
Specificity
Detects human Serpin A7/TBG in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Serpin A9 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Serpin A7/TBG Antibody
Detection of Human Serpin A7/TBG by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Serpin A7/TBG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8176) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Serpin A7/TBG at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Serpin A7/TBG Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human plasma
Sample: Human plasma
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A7/TBG
Serpin A7 (also TBG/Thyroxine-binding globulin and T4-binding globulin) is a secreted 54 kDa glycoprotein, non-inhibitory member of the serpin family of proteins. It is expressed by the liver and secreted in plasma. Mutations in the SERPINA7 gene can result in a whole spectrum of deficiencies, characterized by either reduced or increased TBG levels in the serum. A deficiency in TBG result in low serum total T4 and T3 count in clinically euthyroid subjects in the presence of normal serum TSH. Over aa21-415, human serpin A7 shares 76% aa sequence identity with mouse serpin A7.
Alternate Names
TBG
Gene Symbol
SERPINA7
UniProt
Additional Serpin A7/TBG Products
Product Documents for Human Serpin A7/TBG Antibody
Product Specific Notices for Human Serpin A7/TBG Antibody
For research use only
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