Human Serpin A9/Centerin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5429
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 585302
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A9/Centerin
Ala24-Thr417
Accession # AAQ89063
Ala24-Thr417
Accession # AAQ89063
Specificity
Detects human Serpin A9/Centerin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Serpin A1, A3, A4, A5, A6, A11, A12, B6, B8, B9, C1, D1, E1, E2, F1, F2, G1, I1, I2, recombinant mouse (rm) SerpinA3N, and rmSerpinA9 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human Serpin A9/Centerin Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion-fixed paraffin-embedded sections of human B cell lymphoma
Sample: Immersion-fixed paraffin-embedded sections of human B cell lymphoma
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A9/Centerin
Serpin A9 (Serine proteinase inhibitor-clade A #9; also GCET1, Centerin and Serpin A11) is a 46 kDa, secreted member of the alpha1-antitrypsin (clade A) subfamily, serpin superfamily of protease inhibitors. It is only produced by normal and lymphoma germinal center B cells. Its function is unknown, but it presumably acts as an inhibitor of a trypsin-like protease. Mature human Serpin A9 is presumably 394 amino acids (aa) in length. It contains one Serpin motif (aa 405-415). There are multiple splice variants associated with Serpin A9. There are alternate start sites at Met19, Met99 and Met150. A fourth isoform shows a two aa substitution for aa 369-435, while a fifth isoform shares this same substitution coupled with a 39 aa substitution for aa 1-75. A sixth variant shows a deletion of aa 91-190. Mature human Serpin A9 shares 67% aa identity with mouse Serpin A9.
Long Name
Serpin Peptidase Inhibitor, Clade A, Member 9
Alternate Names
Centerin, GCET1, SERPINA11b
Gene Symbol
SERPINA9
UniProt
Additional Serpin A9/Centerin Products
Product Documents for Human Serpin A9/Centerin Antibody
Product Specific Notices for Human Serpin A9/Centerin Antibody
For research use only
Loading...
Loading...
Loading...
Loading...