Human Serpin A9/Centerin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5429
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A9
Ala24-Thr417
Accession # AAQ89063
Ala24-Thr417
Accession # AAQ89063
Specificity
Detects human Serpin A9 in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Serpin A9/Centerin Antibody
Serpin A9/Centerin in Human Lymphoma.
Serpin A9/Centerin was detected in immersion fixed paraffin-embedded sections of human lymphoma using Human Serpin A9/Centerin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5429) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membranes of lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Serpin A9/Centerin Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymphoma
Sample: Immersion fixed paraffin-embedded sections of human lymphoma
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin A9
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin A9
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A9/Centerin
Serpin A9 (Serine proteinase inhibitor-clade A #9; also GCET1, Centerin and Serpin A11) is a 46 kDa, secreted member of the alpha1-antitrypsin (clade A) subfamily, serpin superfamily of protease inhibitors. It is only produced by normal and lymphoma germinal center B cells. Its function is unknown, but it presumably acts as an inhibitor of a trypsin-like protease. Mature human Serpin A9 is presumably 394 amino acids (aa) in length. It contains one Serpin motif (aa 405-415). There are multiple splice variants associated with Serpin A9. There are alternate start sites at Met19, Met99 and Met150. A fourth isoform shows a two aa substitution for aa 369-435, while a fifth isoform shares this same substitution coupled with a 39 aa substitution for aa 1-75. A sixth variant shows a deletion of aa 91-190. Mature human Serpin A9 shares 67% aa identity with mouse Serpin A9.
Long Name
Serpin Peptidase Inhibitor, Clade A, Member 9
Alternate Names
Centerin, GCET1, SERPINA11b
Gene Symbol
SERPINA9
UniProt
Additional Serpin A9/Centerin Products
Product Documents for Human Serpin A9/Centerin Antibody
Product Specific Notices for Human Serpin A9/Centerin Antibody
For research use only
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