Human Serpin B6 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4336
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin B6
Asp2-Pro376
Accession # P35237
Asp2-Pro376
Accession # P35237
Specificity
Detects human Serpin B6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) Serpin B8 and rhSerpin B9 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human Serpin B6 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Serpin B6
Sample: Recombinant Human Serpin B6
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin B6
Serpin B6 (serine protease inhibitor-clade B#6; also placental thrombin inhibitor) is a 42 kDa, cytosolic member of the ovalbumin (clade B)-subfamily, Serpin superfamily of protease inhibitors. It is produced by multiple cell types (keratinocytes; monocytes; mast cells), and shows inhibitory activity towards thrombin, plasmin and chymotrypsin. Human Serpin B6 is 376 amino acids (aa) in length. An active inhibitor site lies between Glu327 and Met338. Multiple potential isoforms exist. One shows a 28 aa substitution for the N-terminal 191 amino acids. Three others have alternate start sites that vary from 14‑33 aa’s upstream of the standard start site. Human Serpin B6 shares 76% aa identity with mouse Serpin B6.
Alternate Names
CAP, PI6, PTI
Gene Symbol
SERPINB6
UniProt
Additional Serpin B6 Products
Product Documents for Human Serpin B6 Antibody
Product Specific Notices for Human Serpin B6 Antibody
For research use only
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