Human Serpin F1/PEDF Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1177

R&D Systems, part of Bio-Techne
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MAB1177-100
MAB1177-500
MAB1177-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 187003

View all Serpin F1/PEDF Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Serpin F1, Mouse myeloma cell line NS0-derived recombinant human Serpin F1, and S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin F1
Asp44-Pro418
Accession # P36955

Specificity

Detects human Serpin F1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Serpin F1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Serpin F1/PEDF Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin

Western Blot

1 µg/mL
Sample: Recombinant Human Serpin F1/PEDF

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin F1/PEDF

Serpin F1, also known as pigment epithelium derived factor (PEDF), is a secreted glycoprotein that shows sequence and structural similarity with the serpin superfamily proteins, but lacks serine protease inhibitory activity. Serpin F1 has been shown to have cell-type specific functions including inhibition of retinal angiogenesis and neurotrophic activity in the retina and central nervous system.

Long Name

Serpine F1/Pigment Epithelium-derived Factor

Alternate Names

EPC-1, PEDF

Entrez Gene IDs

5176 (Human); 20317 (Mouse); 287526 (Rat)

Gene Symbol

SERPINF1

UniProt

P36955

Additional Serpin F1/PEDF Products

Product Documents for Human Serpin F1/PEDF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Serpin F1/PEDF Antibody

For research use only

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