Human Serum Albumin Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB600-929
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Biotin
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Albumin (Human Serum) (Uniprot: P02768)
Reactivity Notes
Cross reactivity against Human Serum Albumin from other tissues and species may occur but has not been specifically determined
Specificity
Cross reactivity against Albumin from other tissues and species may occur but has not been specifically determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This antibody (Biotin Conjugated) was prepared from monospecific antiserum by immunoaffinity chromatography using purified human serum albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.
This antibody (Biotin Conjugated) was prepared from monospecific antiserum by immunoaffinity chromatography using purified human serum albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.
Scientific Data Images for Human Serum Albumin Antibody [Biotin]
Western Blot: Human Serum Albumin Antibody [Biotin] [NB600-929] - Western Blot of Human Serum Albumin antibody [Biotin]. Lane 1: Human Serum Albumin. Load: 50 ng per lane.Primary antibody: Human Serum Albumin antibody [Biotin] at 1:1,000 overnight at 4C.Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT.Block for 30 min at RT.Predicted/Observed size: 66 kDa, 60 kDa for Human Serum Albumin.
ELISA: Human Serum Albumin Antibody [Biotin] [NB600-929] - ELISA Results of Rabbit Human Serum Albumin antibody [Biotin] tested against purified human serum albumin. Each well was coated in duplicate with 1.0 ug of HSA. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP conjugate stabilizer, Streptavidin-HRP conjugated and TMB substrate.
Applications for Human Serum Albumin Antibody [Biotin]
Application
Recommended Usage
ELISA
1:60000
Immunohistochemistry
1:500-1:3000
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:1000-1:5000
Application Notes
This product has been tested in ELISA, western blot, and suitable in immunohistochemical assays. Optimal concentrations should be determined by researcher.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Polyethylene Glycol (PEG-8000)
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Human Serum Albumin
Alternate Names
albumin, cell growth inhibiting protein 42, DKFZp779N1935, growth-inhibiting protein 20, PRO0883, PRO0903, PRO1341, serum albumin
Gene Symbol
ALB
UniProt
Additional Human Serum Albumin Products
Product Documents for Human Serum Albumin Antibody [Biotin]
Product Specific Notices for Human Serum Albumin Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...