Human Serum Amyloid A4 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3856

R&D Systems, part of Bio-Techne
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AF3856
AF3856-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Serum Amyloid A4 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Serum Amyloid A4
Glu19-Tyr130
Accession # P35542

Specificity

Detects human Serum Amyloid A4 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross‑reactivity with recombinant mouse Serum Amyloid A4 is observed and less than 5% cross-reactivity with recombinant human (rh) Serum Amyloid A1 and rhSerum Amyloid A2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Serum Amyloid A4 Antibody

Serum Amyloid A4 antibody in Human Liver by Immunohistochemistry (IHC-P).

Serum Amyloid A4 in Human Liver.

Serum Amyloid A4 was detected in immersion fixed paraffin-embedded sections of human liver using 15 µg/mL Goat Anti-Human Serum Amyloid A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3856) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Serum Amyloid A4 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

0.1 µg/mL
Sample: Recombinant Human Serum Amyloid A4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serum Amyloid A4

Serum Amyloid A4 (SAA4) is a constitutively expressed SAA protein that is also known as C-SAA. It is an apolipoprotein that constitutes more than 90% of SAA on HDL during homeostasis, and may be involved in lipid transfer between lipoprotein classes. Mature human SAA4 contains 112 amino acid (aa) residues. It is primarily produced in hepatocytes and secreted plasma. In solution, SAA4 is reported to form stable dimers and trimers. Human and mouse SAA4 share 57% aa sequence identity.

Alternate Names

C-SAA, SAA4, SAA5

Entrez Gene IDs

6291 (Human); 20211 (Mouse); 365245 (Rat)

Gene Symbol

SAA4

UniProt

P35542

Additional Serum Amyloid A4 Products

Product Documents for Human Serum Amyloid A4 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Serum Amyloid A4 Antibody

For research use only

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