Human SGPL1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5535
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat
Applications
Validated:
Simple Western, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SGPL1
Lys342-His568
Accession # O95470
Lys342-His568
Accession # O95470
Specificity
Detects human SGPL1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human SGPL1 Antibody
Detection of Human SGPL1 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, HT-29 human colon adenocarcinoma cell line, and HT-29 tumor tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SGPL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5535) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SGPL1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human SGPL1 by Simple WesternTM.
Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SGPL1 at approximately 60 kDa (as indicated) using 50 µg/mL of Goat Anti-Human SGPL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5535) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human SGPL1 Antibody
Application
Recommended Usage
Simple Western
50 µg/mL
Sample: HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line
Sample: HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, HT-29 human colon adenocarcinoma cell line, and HT-29 tumor tissue
Sample: HEK293 human embryonic kidney cell line, HepG2 human hepatocellular carcinoma cell line, HT-29 human colon adenocarcinoma cell line, and HT-29 tumor tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SGPL1
SGPL1 (Sphingosine-1-phosphate lyase; also hSPL) is a 60‑63 kDa member of the SGPL1 subfamily, group II decarboxylase family of enzymes. It is very widely expressed, and particularly concentrated in cells undergoing rapid turnover. SGPL1 is found in the ER, and cleaves phosphorylated sphingoid bases into fatty aldehydes and phosphoethanolamine. This regulates a diversity of cellular processes such as apoptosis and T cell migration. Human SGPL1 is 568 amino acids (aa) in length. It is a type III transmembrane protein (luminal N-terminus with no signal sequence) that contains a 507 aa cytoplasmic domain (aa 62‑568). This domain contains a vitamin B6 binding site (aa 344‑364) that is part of a large catalytic region (aa 116‑506). Over aa 342‑568, human SGPL1 shares 86% aa identity with mouse SGPL1.
Long Name
Sphingosine-1-Phosphate Lyase 1
Alternate Names
SP-lyase, SPL
Gene Symbol
SGPL1
UniProt
Additional SGPL1 Products
Product Documents for Human SGPL1 Antibody
Product Specific Notices for Human SGPL1 Antibody
For research use only
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