Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 5 ng/mL Recombinant Human IFN-a (Catalog # 11100-1) overnight were stained with Mouse Anti-Human Siglec-1/CD169 Monoclonal Antibody (Catalog # MAB5197) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # MAB002).

Applications for Human Siglec-1/CD169 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/mL
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human IFN‑a (Catalog # 11100-1)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-1/CD169

Siglecs are sialic acid specific I-type lectins that belong to the immunoglobulin superfamily. Structurally, they are transmembrane proteins with an N-terminal Ig-like
V‑set domain followed by varying numbers of Ig-like C2-set domains (1, 2). Human Siglec-1, also known as sialoadhesin and CD169, is a 175-185 kDa glycoprotein. It contains a 1622 amino acid (aa) extracellular domain (ECD) with one Ig-like V‑set domain and 16 Ig-like C2-set domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain (3). Within the ECD, human Siglec-1 shares approximately 70% aa sequence identity with mouse and rat Siglec-1. Alternate splicing generates a potentially soluble form of the ECD, and a second isoform with a substituted cytoplasmic domain. Siglec-1 expression is restricted to lymph node and splenic macrophages, plus some tissue macrophages (3). The adhesive function of Siglec-1 is supported by the N-terminal Ig-like domain which shows a selectivity for alpha2,3‑linked sialic acid residues (3‑5). Siglec-1 binds a number of sialylated molecules including the mannose receptor, MGL1, MUC1, PSGL-1, and different glycoforms of CD43 (6‑9). Its binding capacity can be masked by endogenous sialylated molecules (10, 11). The sialylated and sulfated N-linked carbohydrates that modify Siglec-1 itself are required for ligand binding (6, 7). Siglec-1 is expressed on dendritic cells following rhinovirus exposure, and these DC promote T cell anergy (12). It is also induced on circulating monocytes during systemic sclerosis and HIV-1 infection (13‑15). Siglec-1 can trap HIV-1 particles for trans infection of permissive cells (14).

References

Varki, A. and T. Angata (2006) Glycobiology 16:1R.
Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
Hartnell, A. et al. (2001) Blood 97:288.
Nath, D. et al. (1995) J. Biol. Chem. 270:26184.
Crocker, P.R. et al. (1991) EMBO J. 10:1661.
Martinez-Pomares, L. et al. (1999) J. Biol. Chem. 274:35211.
Kumamoto, Y. et al. (2004) J. Biol. Chem. 279:49274.
Nath, D. et al. (1999) Immunology 98:213.
van den Berg, T.K. et al. (2001) J. Immunol. 166:3637.
Nakamura, K. et al. (2002) Glycobiology 12:209.
Barnes, Y.C. et al. (1999) Blood 93:1245.
Kirchberger, S. et al. (2005) J. Immunol. 175:1145.
York, M.R. et al. (2007) Arthritis Rheum. 56:1010.
Rempel, H. et al. (2008) PloS ONE 3:e1967.
van der Kuyl, A.C. et al. (2007) Plos ONE 2:e257.

Long Name

Sialic Acid Binding Ig-like Lectin 1

Alternate Names

CD169, Siglec1

Entrez Gene IDs

6614 (Human); 20612 (Mouse); 311426 (Rat)

Gene Symbol

SIGLEC1

UniProt

Q9BZZ2 (Human)

Additional Siglec-1/CD169 Products

Product Documents for Human Siglec-1/CD169 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Siglec-1/CD169 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Siglec-1/CD169 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5197

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all Siglec-1/CD169 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Siglec-1/CD169 Antibody

Detection of Siglec-1/CD169 in Human PBMCs by Flow Cytometry.