Human Siglec-3/CD33 LlamabodyTM VHH His-tag Antibody
R&D Systems, part of Bio-Techne | Catalog # LMAB10752
Recombinant Monoclonal Antibody. His-tagged VHH domain.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Llama VHH domain Clone # L008.3.28N
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived human Siglec-3/CD33
Asp18-His259
Accession # AAA51948
Asp18-His259
Accession # AAA51948
Specificity
Detects human Siglec-3/CD33 in direct ELISAs.
Clonality
Monoclonal
Host
Llama
Isotype
VHH domain
Scientific Data Images for Human Siglec-3/CD33 LlamabodyTM VHH His-tag Antibody
Detection of Siglec-3/CD33 in HEK293 Human Cell Line Transfected with Human Siglec-3/CD33 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human Siglec-3/CD33 or (B) irrelevant protein and eGFP was stained with Llama Anti-Human Siglec-3/CD33 Llamabody VHH His-tag Monoclonal Antibody (Catalog # LMAB10752) followed by Allophycocyanin-conjugated Anti-His Antibody (IC050A).View our protocol for Staining Membrane-associated Proteins.Detection of Siglec-3/CD33 in Transfected HEK293 Human Embryonic Kidney Cell Line (Positive) And Wild Type HEK293 Human Embryonic Kidney Cell Line (Negative) Cells .
Siglec-3/CD33 was detected in immersion fixed Transfected HEK293 Human Embryonic Kidney Cell Line (Positive) And Wild Type HEK293 Human Embryonic Kidney Cell Line (Negative) Cells using Llama Anti-Human Siglec-3/CD33 LlamabodyTM VHH His-tag Monoclonal Antibody (Catalog # LMAB10752) at 5 µg/mL for 3 hours at room temperature. Cells were stained using Anti-camelid Vhh conjugated to Rhodamine-Red X (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Siglec-3/CD33 LlamabodyTM VHH His-tag Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Siglec-3/CD33 and eGFP
Sample: HEK293 Human Cell Line Transfected with Human Siglec-3/CD33 and eGFP
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed Transfected HEK293 Human Embryonic Kidney Cell Line (Positive) And Wild Type HEK293 Human Embryonic Kidney Cell Line (Negative) Cells
Sample: Immersion fixed Transfected HEK293 Human Embryonic Kidney Cell Line (Positive) And Wild Type HEK293 Human Embryonic Kidney Cell Line (Negative) Cells
Formulation, Preparation, and Storage
Purification
His-tag purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Siglec-3/CD33
References
- Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
- Crocker, P.R. and A. Varki (2001) Immunology 103:137.
- Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
- Simmons, D. and B. Seed (1988) J. Immunol. 141:2797.
- Freeman, S.D. et al. (1995) Blood 85:2002.
- Taylor, V.C. et al. (1999) J. Biol. Chem. 274:11505.
- Ulyanova, T. et al. (1999) Eur. J. Immunol. 29:3440.
Long Name
Sialic Acid Binding Ig-like Lectin 3
Alternate Names
CD33, gp67, Siglec3
Gene Symbol
CD33
UniProt
Additional Siglec-3/CD33 Products
Product Specific Notices for Human Siglec-3/CD33 LlamabodyTM VHH His-tag Antibody
For research use only
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