Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with 10 ng/mL PMA and 200 ng/mL ionomycin for 48 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB1642), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SLAM/CD150 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of SLAM/CD150 in Human Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB1642, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Human SLAM/CD150 ELISA Standard Curve.

Recombinant Human SLAM/CD150 protein was serially diluted 2-fold and captured by Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB1642) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB16421) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human SLAM/CD150 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB16421).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human SLAM/CD150 DuoSet ELISA Kit (Catalog # DY164) for convenient development of a sandwich ELISA.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood lymphocytes

Western Blot

2 µg/mL
Sample: PMA and ionomycin-treated human peripheral blood mononuclear cells (PBMC)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLAM/CD150

Signaling lymphocytic activation molecule (SLAM, SLAMF1; CD150) was the first identified of a family of type I transmembrane (TM) lymphocyte activating receptors. SLAM homotypic adhesion bidirectionally stimulates T and B cells. SLAM is also expressed by hematopoietic stem cells, dendritic cells and platelets and is a T cell measles virus receptor. The 70 kDa glycoprotein contains a 216 amino acid (aa) extracellular domain (ECD) with one C2 type and one V type Ig-like domain, a 20 aa TM sequence and a 76 aa SH2-binding cytoplasmic domain. One splice variant has a shorter cytoplasmic tail and another lacks the TM sequence and is secreted. Human and mouse SLAM ECD share 60% aa identity.

Long Name

Signaling Lymphocytic Activation Molecule

Alternate Names

CD150, IPO-3, SLAMF1

Entrez Gene IDs

6504 (Human); 27218 (Mouse); 102135470 (Cynomolgus Monkey)

Gene Symbol

SLAMF1

UniProt

Q13291

Additional SLAM/CD150 Products

Product Documents for Human SLAM/CD150 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SLAM/CD150 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human SLAM/CD150 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1642

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all SLAM/CD150 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human SLAM/CD150 Antibody

Detection of Human SLAM/CD150 by Western Blot.