Human SLAM/CD150 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF164
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SLAM/CD150
Thr25-Pro237
Accession # Q13291
Thr25-Pro237
Accession # Q13291
Specificity
Detects human SLAM/CD150 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse SLAM, recombinant human (rh) CD48, rhCD84, and rh2B4/CD244 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human SLAM/CD150 Antibody
Detection of Human SLAM/CD150 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SLAM/CD150 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF164) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SLAM/CD150 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of SLAM/CD150 in Human Lymphocytes by Flow Cytometry.
Human whole blood lymphocytes were stained with Sheep Anti-Human SLAM/CD150 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF164, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).Applications for Human SLAM/CD150 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human whole blood lymphocytes
Sample: Human whole blood lymphocytes
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and Nalm-6 human Pre-B acute lymphocytic leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line and Nalm-6 human Pre-B acute lymphocytic leukemia cell line
Reviewed Applications
Read 1 review rated 5 using AF164 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SLAM/CD150
SLAM (also IPO-3 and CD150) is a 70‑80 kDa member of the CD2 family of molecules. It is a costimulatory receptor that mediates T cell activation via SAP. SLAM is found on B cells, dendritic cells, macrophages, stem cells and Th1 T cells. SLAM engages in homophilic interactions. Mature human SLAM is 315 amino acids (aa) in length. It is a type I transmembrane glycoprotein whose extracellular domain contains one V-type and one C2-type Ig-like domain (aa 21‑237). There is a 77 aa SH2‑binding cytoplasmic domain. Four potential splice variants are suggested. One shows a deletion of aa 234‑263 (potentially soluble), while three others show aa substitutions; i.e.‑a ten aa substitution for aa 289‑335, a six aa substitution for aa 264‑335 and a 20 aa substitution for aa 125‑335 (potentially soluble). Over aa 1‑236, human SLAM shares 60% aa identity with mouse SLAM.
Long Name
Signaling Lymphocytic Activation Molecule
Alternate Names
CD150, IPO-3, SLAMF1
Gene Symbol
SLAMF1
UniProt
Additional SLAM/CD150 Products
Product Documents for Human SLAM/CD150 Antibody
Product Specific Notices for Human SLAM/CD150 Antibody
For research use only
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