Human SLC1A5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11628
Key Product Details
| Species Reactivity: | Human |
| Immunogen: |
Synthetic Peptide Accession # Q9JKD8 |
| Applications: | Immunocytochemistry/ Immunofluorescence, Immunohistochemistry |
| Label: | Unconjugated |
| Source: | Recombinant Monoclonal Rabbit IgG Clone # 3085B |
| Specificity: |
Detects a synthetic peptide specific for human SLC1A5 around amino acid 520 in Direct ELISA. |
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ImmunohistochemistryDetection of SLC1A5 in Human Prostate. SLC1A5 was detected in immersion fixed paraffin-embedded sections of human prostate using Rabbit Anti-Human SLC1A5 Monoclonal Antibody (Catalog # mab11628) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
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ImmunocytochemistryDetection of SLC1A5 in SW480 Human Cell Line. SLC1A5 was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Rabbit Anti-Human SLC1A5 Monoclonal Antibody (Catalog # MAB11628) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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