Human SLC31A1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11484

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB11484-100
MAB11484-SP
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2938A

View all SLC31A1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide
Accession # O15431

Specificity

Detects human SLC31A1 in direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human SLC31A1 Antibody

Detection of SLC31A1 in K562 cells by Flow Cytometry

K562 cells were stained with Rabbit Anti-Human SLC31A1 Monoclonal Antibody (Catalog # MAB11484, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Applications for Human SLC31A1 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLC31A1

SLC31A1/CTR1 (solute carrier family 31 member 1/copper transporter 1) is a homotrimeric high-affinity, saturable copper importer implicated in dietary copper uptake. Homeostasis of copper is tightly controlled by inter-regulatory circuitry containing copper, Sp1, and CTR1, wherein Sp1 transcription factor acts as a copper sensor in modulating CTR1 expression which in turn controls cellular copper and Sp1 levels in a 3-way mutual regulatory loop. CTR1 mediated copper uptake is time, temperature, and pH dependent and specific for reduced form of Cu (I) and copper reduction has been proposed to be caused by Steap protein family or Dcytb protein (Cybrd1) both of which are reported to possess cupric reductase activity. The intracellular localization of CTR1 is variable in different cell lines: CTR1 is localized at the plasma membrane in HEK293, CaCo-2, and A2780 cells, whereas, in other cells such as HeLa, it is predominantly localized to vesicular compartments, and variability in trafficking rates between vesicular and plasma membrane compartments has been suggested as the cause of this differential localization. Post‑translational O-linked glycosylation of CTR1 protects it against proteolytic cleavage of N terminal 17-kD fragment and the biological function of this cleaved variant or protease responsible is largely unknown. Selective knockout of Ctr1 in murine intestinal epithelial cells results in severe systemic copper deficiency, ataxia, and death prior to weaning.

Long Name

Solute Carrier Family 31 Member 1

Alternate Names

COPT1, CTR1, HCTR1

Entrez Gene IDs

1317 (Human)

Gene Symbol

SLC31A1

UniProt

O15431 (Human)

Additional SLC31A1 Products

Product Documents for Human SLC31A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SLC31A1 Antibody

For research use only

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