HEK293 human embryonic kidney cell line transfected with (A) human SLC6A4/5-HTTLPR or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human SLC6A4/5-HTTLPR Monoclonal Antibody (Catalog # MAB17292) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human SLC6A4/5-HTTLPR Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human SLC6A4/5-HTTLPR and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLC6A4/5-HTTLPR

SLC6A4 is an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into pre-synaptic neurons. It terminates the action of serotonin and recycles it in a sodium-dependent manner. A repeat length polymorphism in the promoter of SLC6A4 has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients,and depression-susceptibility in people experiencing emotional trauma.

Long Name

Solute Carrier Family 6 Member 4

Alternate Names

5-HT Transporter, 5-HTT, 5-HTTLPR, 5HTTLPR, OCD1, Serotonin Transporter 1, SERT

Entrez Gene IDs

6532 (Human); 15567 (Mouse); 25553 (Rat)

Gene Symbol

SLC6A4

UniProt

P31645 (Human)

Additional SLC6A4/5-HTTLPR Products

Product Documents for Human SLC6A4/5-HTTLPR Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SLC6A4/5-HTTLPR Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human SLC6A4/5-HTTLPR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB17292

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all SLC6A4/5-HTTLPR Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human SLC6A4/5-HTTLPR Antibody

Detection of SLC6A4/5-HTTLPR in HEK293 Human Cell Line Transfected with Human SLC6A4/5-HTTLPR and eGFP by Flow Cytometry.