Human Slit2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB54443

R&D Systems, part of Bio-Techne
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MAB54443-100
MAB54443-SP
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Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 984724

View all Slit2 Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human Slit2
Thr1122-Ser1529
Accession # O94813-1

Specificity

Detects human Slit2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Slit2 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Slit2 Monoclonal Antibody (Catalog # MAB54442).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Reviewed Applications

Read 1 review rated 4 using MAB54443 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Slit2

Slit Homolog 2 (Slit2) is a member of the Slit family of secreted extracellular matrix glycoproteins that are best known for their role in axon guidance (1). It is widely expressed in the developing and adult brain and spinal cord, as well as in fetal lung and kidney, and the adult adrenal gland, thyroid gland, and trachea (1-3). Slit2 is composed of multiple domains including seven EGF-like domains, twenty Leucine-rich repeats (LRRs), one Laminin G-like domain, one C-terminal cysteine knot-like (CTCK) domain, and four N-terminal and four C-terminal LRR domains (1, 3). Slit2 has a molecular weight of approximately 200 kDa (4). However, proteolytic cleavage between the fifth and sixth EGF-like domains produces a membrane-bound 140 kDa N-terminal protein, termed Slit2-N, and a 55-60 kDa C-terminal fragment, termed Slit2-C (4, 5). Mature human Slit2 shares 96% amino acid sequence identity with the mouse and rat orthologs. Slit2 has been shown to have various important functions in the nervous system. Slit2 induces growth cone collapse, inhibits oligodendrocyte precursor cell migration, and promotes axon elongation, branch formation, and fasciculation (5-9). Slit2 C-terminal fragment can mediate axon guidance through binding to Plexin A1 receptor (10). Slit2-C has also been shown to bind to Glypican-1 and promote motor axon migration (5). Outside the nervous system, C-terminal fragment of Slit2 activates a thermogenic PKA pathway in adipocytes and improves glucose homeostasis (11).

References

  1. Ypsilanti, A.R. et al. (2010) Development 137:1939.
  2. Itoh, A. et al. (1998) Brain Res. Mol. Brain Res. 62:175.
  3. Holmes, G.P. et al. (1998) Mech. Dev. 79:57.
  4. Chédotal, A. (2007) Adv. Exp. Med. Biol. 621:65.
  5. Nguyen Ba-Charvet, K.T. et al. (2001) J. Neurosci. 21:4281.
  6. Wang, K.H. et al. (1999) Cell 96:771.
  7. Wong, E.V. et al. (2004) J. Neurobiol. 59:66.
  8. Liu, X. et al. (2012) J. Biol. Chem. 287:17503.
  9. Jaworski, A. and Tessier-Lavigne, M. (2012) Nat. Neurosci. 15:367.
  10. Delloye-Bourgeois, C. et.al. (2014) Nat. Neurosci. 18(1):36.
  11. Svensson, K.J. et al. (2016) Cell Metabolism. 23:1.

Long Name

SLIT Homolog 2

Alternate Names

SLIL3

Entrez Gene IDs

9353 (Human); 20563 (Mouse); 360272 (Rat)

Gene Symbol

SLIT2

UniProt

O94813-1

Additional Slit2 Products

Product Documents for Human Slit2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Slit2 Antibody

For research use only

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