Human SLITRK1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB30091

R&D Systems, part of Bio-Techne
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MAB30091
MAB30091-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 327829

View all SLITRK1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SLITRK1
Ala16-Ser616
Accession # Q96PX8

Specificity

Detects human SLITRK1 in direct ELISAs. In direct ELISAs, no cross-reactivity with reactivity with SLITRK2, 3, 4, or 5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human SLITRK1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLITRK1

SLITRK1 (Slit and Trk-like family member 1) is a charter member of the SLITRK family of proteins (1, 2). This family includes six members, all of which contain a slit‑like extracellular region, and all of which (save for SLITRK1) contain a Trk‑like cytoplasmic region (3). Human SLITRK1 is a type I transmembrane protein 696 amino acids (aa) in length. It contains a 17 aa signal sequence, a 605 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 53 aa cytoplasmic region. The ECD is characterized by the presence of two leucine-rich domains (LRD) that resemble those found in slit. Each LRD (aa 57‑200; aa 374‑517) contains six leucine-rich repeats (LRRs), followed by a Cys-rich, C-terminal LRR (aa 212‑262; aa 529‑579). The exact function of the LRRs is not known. Based on other LRRs, it might be assumed that they mediate protein-protein-interaction (4). There are multiple, single aa mutations in the ECD. Their significance is unclear (5). One truncating frameshift mutation is known that shows a unique 27 aa substitution after Arg 421 (6). This 448 aa form is associated with Tourette’s Syndrome. As noted, the cytoplasmic region is unique among SLITRK family members. It contains no known signaling motifs, which is in contrast to SLITRK2‑6 which show TrkA-like tyrosine phosphorylation sites. This difference is reflected in their function. SLITRK1 is a protein that is found in adult neurons of the cerebrum, thalamus and hippocampus. It induces neurite outgrowth whereas SLITRK2‑6 block neurite extension (3). Human SLITRK1 shares 99%, 98% and 99% aa sequence identity with canine, mouse and bovine SLITRK1, respectively.

References

  1. Aruga, J. et al. (2003) Gene 315:87.
  2. Nagase, T. et al. (2001) DNA Res. 8:179.
  3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.
  4. Enkhbayar, P. et al. (2003) Proteins 54:394.
  5. Zuchner, S. et al. (2006) Mol. Psychiat. 11:888.
  6. Abelson, J.F. et al. (2005) Science 310:317.

Long Name

SLIT and NTRK-like Family, Member 1

Alternate Names

LRRC12

Entrez Gene IDs

114798 (Human); 76965 (Mouse)

Gene Symbol

SLITRK1

UniProt

Q96PX8

Additional SLITRK1 Products

Product Documents for Human SLITRK1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SLITRK1 Antibody

For research use only

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