Human Smad4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB20971
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # 253327
Product Specifications
Immunogen
E. coli-derived recombinant human Smad4
Pro139-Asp332
Accession # Q13485
Pro139-Asp332
Accession # Q13485
Specificity
Detects endogenous human Smad4 in Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human Smad1, 5, 6, 7, or 9.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Scientific Data Images for Human Smad4 Antibody
Detection of Human Smad4 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Human Smad4 Monoclonal Antibody (Catalog # MAB20971) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Smad4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Smad4 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and HEK293 human embryonic kidney cell line
Sample: HepG2 human hepatocellular carcinoma cell line and HEK293 human embryonic kidney cell line
Reviewed Applications
Read 1 review rated 5 using MAB20971 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Smad4
Long Name
Mothers Against DPP Homolog 4
Alternate Names
DPC4, MADH4
Entrez Gene IDs
4089 (Human)
Gene Symbol
SMAD4
UniProt
Additional Smad4 Products
Product Documents for Human Smad4 Antibody
Product Specific Notices for Human Smad4 Antibody
For research use only
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