Smoothelin B was detected in formalin fixed paraffin-embedded sections of human colon using Mouse Anti-Human Smoothelin B Monoclonal Antibody (Catalog # MAB8278) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells in vasculature. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Smoothelin in HASMC Human Cells by Flow Cytometry.

HASMC human aortic smooth muscle cells was stained with Mouse Anti-Human Smoothelin B Monoclonal Antibody (Catalog # MAB8278, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Intracellular Molecules.

Applications for Human Smoothelin B Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: HASMC human aortic smooth muscle cells

Reviewed Applications

Read 1 review rated 1 using MAB8278 in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Smoothelin

Smoothelin (SMTN) is a cytoskeletal protein associated with stress fibers. It is 917 amino acids (aa) in length and shares 77% and 82% aa identity with mouse and rat SMTN. SMTN has two major isoforms, A and B with reported molecular weights of approximately 59 and 110 kDa, respectively. Isoform A is associated with visceral smooth muscle cells while isoform B is specific to vascular smooth muscle. Loss of SMTN has been associated with defects in intestinal smooth muscle cell contraction as well as increased arterial pressure and cardiac hypertrophy.

Alternate Names

SMSMO, SMTN

Entrez Gene IDs

6525 (Human)

Gene Symbol

SMTN

UniProt

P53814

Additional Smoothelin Products

Product Documents for Human Smoothelin B Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Smoothelin B Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Smoothelin B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8278

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Smoothelin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Smoothelin B Antibody

Smoothelin B in Human Colon.