Human SMURF1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9507
Key Product Details
Species Reactivity
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 530131
Product Specifications
Immunogen
E. coli-derived recombinant human SMURF1
Met496-Glu757
Accession # Q9HCE7
Met496-Glu757
Accession # Q9HCE7
Specificity
Detects human SMURF1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human SMURF1 Antibody
Detection of Human SMURF1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SMURF1 Monoclonal Antibody (Catalog # MAB9507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SMURF1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human SMURF1 by Simple WesternTM.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SMURF1 at approximately 82 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human SMURF1 Monoclonal Antibody (Catalog # MAB9507) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human SMURF1 Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SMURF1
Long Name
SMAD Ubiquitination Regulatory Factor 1
Alternate Names
EC 6.3.2, KIAA1625
Gene Symbol
SMURF1
UniProt
Additional SMURF1 Products
Product Documents for Human SMURF1 Antibody
Product Specific Notices for Human SMURF1 Antibody
For research use only
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