Human SNF1LK2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5737
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SNF1LK2
Ala663-Leu818
Accession # Q9H0K1
Ala663-Leu818
Accession # Q9H0K1
Specificity
Detects human SNF1LK2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human SNF1LK2 Antibody
SNF1LK2 in Human Adipocytes.
SNF1LK2 was detected in immersion fixed human adipocytes using 10 µg/mL Goat Anti-Human SNF1LK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5737) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human SNF1LK2 by Western Blot.
Western blot shows lysates of human mesenchymal stem cells (MSC) grown in the absence (-) or presence (+) of StemXVivo™ Adipogenic Supplement (Catalog # CCM001). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SNF1LK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5737), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SNF1LK2 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human SNF1LK2 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human adipocytes
Sample: Immersion fixed human adipocytes
Western Blot
1 µg/mL
Sample: Human mesenchymal stem cells (MSC) cultured with StemXVivo™ Adipogenic Supplement (Catalog # CCM001)
Sample: Human mesenchymal stem cells (MSC) cultured with StemXVivo™ Adipogenic Supplement (Catalog # CCM001)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SNF1LK2/SIK2
Long Name
SNF1-like Kinase 2
Alternate Names
LOH11CR1I, QIK, SIK2
Gene Symbol
SIK2
UniProt
Additional SNF1LK2/SIK2 Products
Product Documents for Human SNF1LK2 Antibody
Product Specific Notices for Human SNF1LK2 Antibody
For research use only
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