Human SorCS2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4238

R&D Systems, part of Bio-Techne
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AF4238
AF4238-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all SorCS2 Antibodies »

Product Specifications

Immunogen

NS0-derived recombinant human SorCS2
Ser70-Gly1078
Accession # Q96PQ0

Specificity

Detects human SorCS2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse (rm) SorCS2 is observed and less than 1% cross-reactivity with recombinant human (rh) SorCS1 and rhSorCS3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human SorCS2 Antibody

Detection of Human SorCS2 antibody by Western Blot.

Detection of Human SorCS2 by Western Blot.

Western blot shows lysates of human kidney (medulla) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human SorCS2 Antigen Affinity-purified Antibody (Catalog # AF4238) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SorCS2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
SorCS2 antibody in Human Brain by Immunohistochemistry (IHC-P).

SorCS2 in Human Brain.

SorCS2 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Sheep Anti-Human SorCS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4238) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and their processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human SorCS2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (medulla)

Western Blot

1 µg/mL
Sample: Human kidney (medulla) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SorCS2

SorCS2 is a type I transmembrane glycoprotein receptor that belongs to the mammalian Vps10p (vacuolar protein-sorting 10 protein) family (1, 2). Family receptors include sortilin, SorLA, and three SorCS proteins. All SorCS proteins are predominantly expressed in the brain, especially during development, but vary in other locations (1‑3). SorCS2 mRNA has also been found in the kidney, lung, testis and heart (1, 2). SorCS2 presumably mediates endocytosis, but its ligands are not well‑defined. However, the other SorCS proteins have been shown to bind NGF and PDGF-BB (4‑6). Human SorCS2 is synthesized as a 1159 amino acid (aa) prepro form with a 50 aa signal sequence and a potential furin-type proteolytic processing site at aa 119. This would produce a mature SorCS2 protein of 1040 aa with a 959 aa extracellular/lumenal domain (ECD), a 21 aa transmembrane domain and a 60 aa cytoplasmic domain. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain (2). Within the ECD, human SorCS2 shares 89%, 88%, 88% and 79% aa identity with mouse, rat, equine and canine SorCS2, respectively. It also shares 46% aa identity with the ECD of both SorCS1 and SorCS3. Unlike other SorCS, shedding of the SorCS2 ECD occurs very slowly and is mainly independent of the metalloproteinase TACE/ADAM17 (4).

References

  1. Hampe, W. et al. (2001) Hum. Genet. 108:529.
  2. Rezgaoui, M. et al. (2001) Mech. Dev. 100:335.
  3. Hermey, G. et al. (2004) J. Neurochem. 88:1470.
  4. Hermey, G. et al. (2006) Biochem. J. 395:285.
  5. Westergaard, U. et al. (2004) J. Biol. Chem. 279:50221.
  6. Westergaard, U. B. et al. (2005) FEBS Lett. 579:1172.

Long Name

Sortilin-related VPS10 Domain Containing Receptor 2

Alternate Names

KIAA1329VPS10 domain receptor protein, sortilin-related VPS10 domain containing receptor 2, VPS10 domain-containing receptor SorCS2

Entrez Gene IDs

57537 (Human); 81840 (Mouse)

Gene Symbol

SORCS2

UniProt

Q96PQ0

Additional SorCS2 Products

Product Documents for Human SorCS2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human SorCS2 Antibody

For research use only

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