Human SOX15 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4070
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SOX15
Met1-Leu233
Accession # O60248
Met1-Leu233
Accession # O60248
Specificity
Detects human SOX15 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) SOX3, rhSOX14, and rhSOX21 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human SOX15 Antibody
Detection of Human SOX15 by Western Blot.
Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line and BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SOX15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4070) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SOX15 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SOX15 in Human iPSK3 cells.
SOX15 was detected in immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3) using Sheep Anti-Human SOX15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4070) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
SOX15 in A431 Human Cell Line.
SOX15 was detected in immersion fixed A431 human epithelial carcinoma cell line (positive staining) and HUVEC human umbilical vein endothelial cells (negative staining) using Sheep Anti-Human SOX15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4070) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human SOX15 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line and human plasmid-derived induced pluripotent stem cells (iPSK3) and A431 human epithelial carcinoma cell line
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line and human plasmid-derived induced pluripotent stem cells (iPSK3) and A431 human epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: JEG-3 human epithelial choriocarcinoma cell line andBG01V human embryonic stem cells
Sample: JEG-3 human epithelial choriocarcinoma cell line andBG01V human embryonic stem cells
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX15
Long Name
Transcription Factor SOX15
Alternate Names
SOX20, SOX26, SOX27
Gene Symbol
SOX15
UniProt
Additional SOX15 Products
Product Documents for Human SOX15 Antibody
Product Specific Notices for Human SOX15 Antibody
For research use only
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